The Flow Cytometry Core at Penn State College of Medicine recently acquired a new instrument for researchers. The ImageStreamX Mark II Imaging Flow Cytometer combines the speed, sensitivity and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.
The new instrument produces multiple high-resolution images of every cell directly in flow, including brightfield and darkfield (SSC), and up to 10 fluorescent markers with sensitivity exceeding conventional flow cytometers.
The ImageStream will acquire up to 12 images simultaneously of each cell or object including brightfield, scatter, and multiple fluorescent images at rates of up to 5,000 objects per second with high photonic sensitivity.
Applications include the study of cell-to-cell interactions, phagocytosis, apoptosis and autophagy, the characterization of circulating tumor cells, and many others.
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